Yes.
The preferred method is to use opposite ends of the battery bank..... so as above you would take the main positive from battery A and main negative from battery B..... though this more applies to larger UPS/Boat/larger battery banks.
If the batteries are next to each other and the interconnected cables are short and decent gauge it wont realy make mu h difference.
The most important thing is over gauge the cable . . . . Fatter the better.
You already have a ctek 20A charge . . . So the assumption would be that the existing feed cable is suitable....
The factory setup comes with a 100A capable cable and down fused to 80A.... you will need to check your feed cables to make sure they can carry a sustained 20A current from the ctek without heating up etc. (When they leisure batteries are low they will easily draw up to the maximum the source can supply.. in your case 20A fron your 20A ctek. Or over 50A in the case of a vsr. Or 40A in the case of my redarc bcdc1240)
Again larger the cable the better..... even if its fused much lower.
id go way larger . . .
maybe just get a wire kit like this . . . https://www.travelvolts.net/product-page/wiring-kit-for-ripca-elogic-el1225
what are your loads on the battery bank?
remember that 20A ctek is just a charging current . . . . . . but if you connect an inverter or other to the battery bank it may draw 20A-100A (which will blow that 20A interconnect fuse)
so your interconnect cable and fuses etc need to be good enough for your total expected load>>>>
ie 12v socket - 10A
led light 1-10A
inverter 1-100A
etc
etc etc
i use 100A cable for the feeds and grounds for the battery bank. . . . . and then use smaller cable for all the outgoing circuits.
more info :
Installing 12v Socket /s - How Its Done -